Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

dksA - DNA-binding transcriptional regulator of...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0144, JW0141, msmA

Mogull, S.A. et al., Paul, B.J. et al., Paul, B.J. et al., Dubois, D.Y. et al., Yamanaka, K. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute by deleting incorrect information, restructuring or completely rewriting any text.

Disease relevance of dksA

Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue [1].
The yadB gene is expressed in wild-type E. coli as an operon with the dksA gene, which encodes a protein involved in the general stress response by means of its action at the translational level [2].
dksA is required for intercellular spread of Shigella flexneri via an RpoS-independent mechanism [3].

High impact information on dksA

In vitro, DksA binds to RNAP, reduces open complex lifetime, inhibits rRNA promoter activity, and amplifies effects of ppGpp and the initiating NTP on rRNA transcription, explaining the dksA requirement in vivo [4].
Consistent with the model that ppGpp/DksA stimulates amino acid promoters both directly and indirectly in vivo, cells lacking dksA fail to activate transcription from the hisG promoter after amino acid starvation [5].
To identify proteins interacting with the MukB protein, we isolated three multicopy suppressors (msmA, msmB, and msmC) to the temperature-sensitive colony formation of the mukB106 mutation [6].
The S. flexneri dksA mutant exhibited sensitivity to acid and oxidative stress, in part due to an effect of DksA on production of RpoS [3].
In this study, we identified dksA as a gene necessary for intercellular spread in, but not invasion of, cultured cells [3].

Biological context of dksA

Sequenced spontaneous extragenic suppressors of dksA polyauxotrophy are frequently the same T563P rpoB allele that suppresses a ppGpp(0) phenotype [7].
Another multicopy suppressor gene was identified as the dksA gene [8].
DNA sequence analysis and in vivo experiments showed that dksA encodes a 17,500-Mr polypeptide [9].
Gene disruption experiments indicated that dksA is not an essential gene [9].

Other interactions of dksA

With the exception of the dksA mutant, which had a modest defect in Luria-Bertani medium, none of these mutants was defective for rpoS stationary-phase induction [10].
The nucleotide sequence of the suppressor revealed that the suppressor gene was identical to dksA, which encodes a multicopy suppressor of the heat shock gene dnaK [11].

Analytical, diagnostic and therapeutic context of dksA

Cloning and Northern blot analysis revealed that dksA was neither mutated nor less transcribed in mutant PR1-E4 [1].

References

Inhibition of quorum sensing by a Pseudomonas aeruginosa dksA homologue. Branny, P., Pearson, J.P., Pesci, E.C., Köhler, T., Iglewski, B.H., Van Delden, C. J. Bacteriol. (2001)
An aminoacyl-tRNA synthetase-like protein encoded by the Escherichia coli yadB gene glutamylates specifically tRNAAsp. Dubois, D.Y., Blaise, M., Becker, H.D., Campanacci, V., Keith, G., Giegé, R., Cambillau, C., Lapointe, J., Kern, D. Proc. Natl. Acad. Sci. U.S.A. (2004)
dksA is required for intercellular spread of Shigella flexneri via an RpoS-independent mechanism. Mogull, S.A., Runyen-Janecky, L.J., Hong, M., Payne, S.M. Infect. Immun. (2001)
DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP. Paul, B.J., Barker, M.M., Ross, W., Schneider, D.A., Webb, C., Foster, J.W., Gourse, R.L. Cell (2004)
DksA potentiates direct activation of amino acid promoters by ppGpp. Paul, B.J., Berkmen, M.B., Gourse, R.L. Proc. Natl. Acad. Sci. U.S.A. (2005)
Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli. Yamanaka, K., Mitani, T., Ogura, T., Niki, H., Hiraga, S. Mol. Microbiol. (1994)
DksA affects ppGpp induction of RpoS at a translational level. Brown, L., Gentry, D., Elliott, T., Cashel, M. J. Bacteriol. (2002)
Deletion of the yhhP gene results in filamentous cell morphology in Escherichia coli. Ishii, Y., Yamada, H., Yamashino, T., Ohashi, K., Katoh, E., Shindo, H., Yamazaki, T., Mizuno, T. Biosci. Biotechnol. Biochem. (2000)
Identification and characterization of a new Escherichia coli gene that is a dosage-dependent suppressor of a dnaK deletion mutation. Kang, P.J., Craig, E.A. J. Bacteriol. (1990)
Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli. Hirsch, M., Elliott, T. J. Bacteriol. (2002)
A suppressor of mutations in the region adjacent to iterons of pSC101 ori. Ohkubo, S., Yamaguchi, K. J. Bacteriol. (1997)

[search][advanced]